Seasonal Dynamics of the Antioxidant Enzymes Activities in Blood and the Content of Toxic Elements in the Muscle of the Knout Goby from the Black Sea

About the authors:

Tatyana B. Kovyrshina, Researcher, Ecotoxicological Laboratory, A. O. Kovalevsky Institute of Marine Biological Research of the RAS (Sevastopol); mtk.fam@mail.ru

Svetlana O. Omelchenko, Cand. Sci. (Biol.), Senior Lecturer, Department of Natural Sciences and Mathematics Education, Crimean Republican Institute of Post-Diploma Pedagogical Education (Simferopol); svet.omelchenko@mail.ru

Abstract:

This article studies the seasonal dynamics of the antioxidant enzymes activities in blood and the content of toxic elements in the muscles of knout goby (Mesogobius batrachocephalus Pallas) from the coastal waters of Sevastopol (the Black Sea). The authors discuss the influence of toxic elements contained in muscles on the activities of antioxidant enzymes in blood of knout goby. The obtained results show that the most sensitive to tissue accumulation of toxic elements are enzymes CAT (0.95, −0.52 < r < −0.97) and GR (0.96, −0.56 < r < −0.93), while Cu, Hg and As have the most toxic effect on the biochemical parameters.

References:

1. Borvinskaya E. V., Smirnov L. P., N. Nemova N. 2009. “Glutation-S-transferazy ryb — potentsial’nye ekologo-biokhimicheskie indikatory antropogennogo vozdeystviya na vodnuyu sredu (obzor)” [Glutathione-S-Transferase of Fish — Potential Ecological and Biochemical Indicators of Anthropogenic Impact on the Aquatic Environment (Review)]. Trudy Karel’skogo nauchnogo tsentra RAN, no 3, pp. 8-19.
2. Vaganov A. S. 2011. “Sravnitel’naya kharakteristika soderzhaniya tyazhelykh metallov v promyslovykh vidakh ryb kuybyshevskogo vodokhranilishcha” [Comparative Characteristics of Heavy Metals in Commercial Fish Species of the Kuibyshev Reservoir]. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk, vol. 13, no 5 (2), pp. 143-146.
3. Vasil’eva E. D. 2007. Ryby Chernogo morya. Opredelitel’ morskikh, solonovatovodnykh, evrigalinnykh i prokhodnykh vidov s tsvetnymi illyustratsiyami, sobrannykh S. V. Bogorodskim [Pisces of the Black Sea. The Determinant of Marine, Brackish Water, Euryhaline and Pass-Through Species with Color Illustrations Collected by S. V. Bogorodsky]. Moscow: Izd-vo VNIRO. 
4. Golovanova I. L. 2008. “Vliyanie tyazhelykh metallov na fiziologo-biokhimicheskiy status ryb i vodnykh bespozvonochnykh” [The Influence of Heavy Metals on the Physiological and Biochemical Status of Fish and Aquatic Invertebrates]. Biologiya vnutrennikh vod, no 1, pp. 99-108.
5. Golovanova I. L., Filippov A. A., Krylov V. V., Chebotareva Yu. V., Izyumov Yu. G. 2013. “Deystvie magnitnogo polya i medi na aktivnost’ gidroliticheskikh fermentov u segoletok plotvy Rutilus rutilus” [Effect of Magnetic Field and Copper on the Activity of Hydrolytic Enzymes in Roach Rutilus rutilus]. Voprosy ikhtiologii, vol. 53, no 2, pp. 227-232. DOI: 10.7868/S0042875213020045
6. Kovyrshina T. B., Omel’chenko S. O. 2014. “Sezonnye izmeneniya soderzhaniya toksichnykh elementov i parametrov okislitel’nogo stressa v tkanyakh chernomorskogo bychka-kruglyaka Neogobius melanostomus” [Seasonal Changes in the Content of Toxic Elements and Oxidative Stress Parameters in the Tissues of the Black-Headed Goby Neogobius melanostomus]. Vestnik Sankt-Peterburgskogo universiteta. Seriya 3. Biologiya, no 1, pp. 14-23.
7. Kovyrshina T. B., Rudneva I. I. 2016. “Vliyanie zagryazneniya pribrezhnykh vod Chernogo morya na biomarkery krovi bychka-kruglyaka Neogobius melanostomus Pallas, 1811 (Perciformes: Gobiidae)” [Impact of Pollution of the Black Sea’s Coastal Waters on the Biomarkers of the Blood of the Bull-Calf Neogobius melanostomus Pallas, 1811 (Perciformes: Gobiidae)]. Biologiya morya, vol. 42, no 1, pp. 34-40.
8. Kopytov Yu. P., Minkina N. I., Samyshev E. Z. 2010. “Uroven’ zagryaznennosti vody i donnykh otlozheniy Sevastopol’skoy bukhty (Chernoe more)” [The Level of Pollution of Water and Bottom Sediments of the Sevastopol Bay (Black Sea)]. In: Sistemy kontrolya okruzhayushchey sredy. Sb. nauch. tr. MGI NAN Ukrainy [Environmental Monitoring Systems], no 14, pp. 199-208. Sevastopol.
9. Lakin G. F. 1990. Biometriya [Biometrics]. Moscow: Vysshaya shkola.
10. Naydenova N. N. 1976. “Zavisimost’ parazitofauny bychkov ot sezona goda” [Dependence of the Parasite Fauna of Knout Gobys on the season]. Biologiya morya, no 36, pp. 91-96.
11. Omelchenko S. O. 2009. “Sostoyanie azotistogo obmena ryb v usloviyakh zagryazneniya nitrozaminami i toksichnymi elementami” [The State of Nitrogen Metabolism of Fish in Conditions of Contamination with Nitrosamines and Toxic Elements]. Cand. Sci. (Biol.) diss. abstract. Simferopol.
12. Poduvkin N. A., Smutnev P. V. 2017. “Osobennosti svobodnoradikal’nykh protsessov v organizme nekotorykh vidov khishchnykh ryb v razlichnye sezony goda” [Features of Free-Radical Processes in the Organism of Some Species of Predatory Fish in Different Seasons]. Vestnik AGTU. Ser. Rybnoe khozyaystvo, no 1, pp. 117-123. 
13. SanPiN 2.3.2.560-96 1996 “Gigienicheskie trebovaniya k kachestvu i bezopasnosti prodovol’stvennogo syr’ya i pishchevykh produktov” [Hygienic Requirements for the Quality and Safety of Food Raw Materials and Food Products]. Approved by RF Goskomsanepidnadzor on 24 October, no 27.
14. Svetovidov A. N. 1964. Ryby Chernogo morya [Pisces of the Black Sea]. Leningrad: Nauka. 
15. Jezierska B., Lugovska K., Witeska M. 2009. “The Effects of Heavy Metals on Embryonic Development of Fish (A Review)”. Fish Physiology and Biochemistry, vol. 35, no 4, pp. 625-640. DOI: 10.1007/s10695-008-9284-4
16. Almroth B. C., Sturve J., Stephensen E., Holth T. F., Fӧrlin L. 2008. “Protein Carbonyls and Antioxidant Defenses in Corkwing Wrasse (Symphodus melops) from a Heavy Metal Polluted and a PAH Polluted Site”. Marine Environmental Research, vol. 66 (2), pp. 1-29. DOI: 10.1016/j.marenvres.2008.04.002