Release:2016, Vol. 2. №2
About the authors:Svetlana S. Kolyvanova, Junior Researcher, Department of Bioresources Сryosphere, Tyumen Scientific Centre of the Siberian Branch of the Russian Academy of Sciences; Рostgraduate Student, Department of Anatomy and Рhysiology of Нumans and Аnimals, University of Tyumen; email@example.com
Abstract:The effect of metabolites of 14 strains of the microorganisms isolated from the permafrost of West (near Tarko-Sale) and Eastern (Chara depression Chita Oblast) Siberia and Central Yakutia (Mamontova mountain) on the proliferative activity of peripheral blood lymphocytes (0(I)Rh+)) in the reaction of blast-cell transformation. The authors consider the number of blast-cell transformed lymphocytes in the spontaneous blast-cell transformation under the influence of mitogen for T-lymphocytes of phytohemagglutinin (PHA) and under microbial metabolites of permafrost of PHA + metabolites complex. It is found that different strains of the permafrost microorganisms can have both stimulatory and suppressive effect on lymphocyte proliferation. The strain F2 from Central Yakutia permafrost is exerting maximum stimulating effect (p<0.01), minimum — strains 9/48 (II) and 5/26 from Western Siberia permafrost (p <0.05). Strain F2 is increasing the level of lymphocyte proliferation in combination with PHA (p<0.05). Two strains of Western Siberia permafrost — 9/48 (II) and 3/07 (p<0.01 in both cases) and two strains of Eastern Siberia permafrost — 6/010 and 2/09 (p<0.01 in both cases) are significantly reducing PHA-stimulated lymphocyte proliferation.