Effect of steroid oxidative stress on morphofunctional organization of tissue compartments maintaining mineral homeostasis

About the authors:

Natalia N. Lukina, Student, Department of Ecology and Genetics, Institute of Biology, Tyumen State University
Andrei V. Sakharov, Professor, Head of the department of Zoology and Methods of Teaching biology, Novosibirsk State Pedagogical University, Novosibirsk
Alexander E. Prosenko, Dr. Sci. (Chem.), Professor, Head of the Department of Chemistry, Novosibirsk State Pedagogical University
Lyubov N. Sivokhina,
Sofia V. Oleinikova ,

Abstract:

The influence of glucocorticosteroid-induced oxidative stress on the tissue compartments regulating mineral homeostasis was studied with morphology methods. It is found that the prolonged use of glucocorticosteroids leads to morphofunctional mucosa disturbances of the distal colon, renal glomerular apparatus and nephrothelial and bone tissue of the vertebral bodies. The morphological features of rats’ intestine and kidney cells recognize the leading role of a free radical mechanism in their damage. In colon mucosa, the lethal damage of colonocytes is developing by apoptosis mechanism. In kidneys of the rats that have being receiving prednisolone for a long time, we found a differential level of response of the cortical and juxtamedullary nephrons on chronic hormonal load. Pathological changes are most pronounced in the cortical nephrons compared with juxtamedullary. The latter are more resistant to damage by activated oxygen metabolites. Reduced bone mass of the vertebral body due to thinning of bone beams and increased osteoclastic resorption of bone matrix cortical plate of the vertebral body shows that prolonged injection of prednisolone leads to disruption of the processes of modeling and remodeling bone.The totality of the detected changes in the tissues of examined organs suggests that the supply of activated oxygen metabolites in the tissue compartments under glucocorticosteroid-induced oxidative stress is carried out mainly hematogenously.

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