The influence of membrane skeleton solubilized proteins of erythrocytes on the activity of na, k-at pase

About the authors:

Alexander D. Shalabodov, Dr. Biol. Sci., Professor, Director at the Institute of Mathematics, Natural Sciences and Information Technologies, Tyumen State University
Marina N. Maslova, Doct. Sci. (Biol.), Professor, Leading Researcher, I. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Dmitry N. Kyrov, Cand. Sci. (Biol.), Associate Professor, Department of Human and Animal Anatomy and Physiology, Tyumen State University

Abstract:

The influence of membrane skeleton solubilized proteins of denuclearized erythrocytes on the activity of Na, K-AT Pase is studied. It is shown that SPMS obtained using the medium not containing chelators activate Na, K-ATPase of the rat brain, whereas the calcium ions do not change the activating effect of SPMS. The introduction of SPMS EDTA or magnesium ions into the receiving medium allows to save a calcium-dependent modulating influence of SPMS on the activity of the Na, K-AT Pase of the brain as well as the activity of the membrane preparations of rats´ erythrocytes. Depending on the method of the preparation of erythrocyte ghosts and SPMS, one may observe both activation and inhibition of Na, K-ATPase via removed membrane proteins. Yet, the presence of Ca2+ ions in the incubation medium alters the activation of Na, K-ATPase via the membrane skeleton solubilized proteins. It is supposed that a mixture of membrane skeleton solubilized proteins of denuclearized erythrocytes contains the Mg2+, Ca2+ factor(s), modulating the activity of the Na, K-ATPase.

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