Platelet chemiluminescence. Application of chemiluminescence method in platelet activity definition

About the authors:

Lyudmila V. Krivokhizhina, Dr. Med. Sci., Professor, Department of
Pathophysiology, South Ural State Medical University

Salavat A. Kantyukov, Cand. Med. Sci., Associate Professor, Department of
Biochemistry, South Ural State Medical University

Elena N. Yermolaeva, Cand. Med. Sci., Associate Professor, Department of
Pathophysiology, South Ural State Medical University

Dmitri N. Krivokhizhin, Head of the Department of Purulent Surgery, City
Clinical Hospital № 1 (Chelyabinsk)

Abstract:

Platelets both in intact and in activated condition are capable of generation of active forms of oxygen (AFO). Production of AFO by platelets of humans and rats is proved. Intensity of AFO generation can be registered with the help of chemiluminescence method (HL). The method is based on addition of luminol into platelet-rich plasma (PRP) and chemiluminescence registration with chemiluminometer HL-003 with a graphical display of chemiluminogram on the computer monitor. Addition of the physiological inducer of aggregation ADP (adenosine diphosphate) in the dose used for laboratory evaluation of platelet aggregation more than twice increases emission. For rats, due to the specific nature of platelet aggregation, it is necessary to add CaCl2 (1,0 mM) into the sample together with ADP. Thus, the platelet chemiluminescence method allows to estimate the functional condition of intact (HL in the presence of only luminal) and activated platelets (HL in the presence of luminal and ADF) according to their ability to produce active metabolites of oxygen.

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