Experimental studies of himic compounds biodegradation induced by fungal enzymatic oxidizing complex

About the authors:

Tatyana N. Gubernatorova, Cand. Techn. Sci., Senior Researcher, Water Problems Institute, Russian Academy of Sciences
Marina I. Dinu, Cand. Chem. Sci, research assistant, Institute of Geochemistry and Analytical Chemistry, named after V.I. Vernadskiy, Russian Academy of Sciences Vitaliy Y. Khoroshavin, Cand. Geog. Sci., Head of Department of Physical Geography and Ecology, Institute of Mathematics, Natural Sciences and Information Technologies, Tyumen State University

Abstract:

Degradation of humic substances in water environment induced by fungal enzymatic oxidizing complex is a natural biochemical process, occurring in water ecosystems. Humic substances are persistent organic compounds which are slowly decomposed and inclined to polymerization processes. Biodegradation of humic substances induced by fungi is a very common process and plays an important role in the environment. Unfortunately, these processes are poorly investigated. In the present work we studied the biodegradation processes of humic substances induced by the fungal enzymatic oxidizing complex. The manganese peroxidase from Phanerochaete chrysosporium and peroxidase from Bjerkandera adusta was chosen as a typical oxidizing enzyme of the ligninolytic complex of fungi-decomposers. During the experiment we studied the impact of the enzymes on humic substances, when placed directly. Absorbance variations and infrared spectrum changes were estimated in the prepared samples by spectrometric analysis techniques. We also measured changes of the weight-average molecular weight of humic acid and COD values, followed by the data analysis and comparison of obtained results.

References:

1. Middelburg, J.J. A simple rate model for organic matter decomposition in marine sediments. Geochim. Cosmochim. Acta. 1989. V. 53. Pp. 1577-1581.

2. Boudreau, B.P., Ruddick, B.R. On a reactive continuum representation of organic matter diagenesis. Amer. J. Sci. 1991. V. 291. Pp. 507-538.

3. Dolgonosov, B.M., Gubernatorova, T.N. Mekhanizmy i kinetika destruktsii organicheskogo veshchestva v vodnoi srede [Degradation Mechanisms and Kinetics of Organic Matter in an Aquatic Environment]. Мoscow, 2011. 208 p. (in Russian).

4. Dolgonosov, B.M., Gubernatorova, T.N. Modeling the Biodegradation of Multicomponent Organic Matter in an Aquatic Environment: 1. Methodology. Water Resources. 2010. Vol. 37. № 3. Pp. 311–319.

5. Dolgonosov, B.M., Gubernatorova, T.N. Modeling the Biodegradation of Multicomponent Organic Matter in an Aquatic Environment: 2. Analysis of the Structural Organization of Lignin. Water Resources. 2010. Vol. 37. № 3. Pp. 320–331.

6. Gubernatorova, T.N., Dolgonosov, B.M. Modeling the Biodegradation of Multicomponent Organic Matter in an Aquatic Environment: 3. Analysis of Lignin Degradation Mechanisms. Water Resources. 2010. Vol. 37. № 3. Pp. 332–346.

7. Dolgonosov, B.M., Gubernatorova, T.N. Modeling the Biodegradation of Multicomponent Organic Matter in an Aquatic Environment: 4. Degradation Kinetics Model. Water Resources. 2010. Vol. 37. № 3. Pp. 347–360.

8. Dinu, M.I. Vliianie funktsional'nykh osobennostei gumusovykh veshchestv na formy nakhozhdeniia metallov v prirodnykh vodakh [The influence of humic substances functional characteristics on the deportment of metals in natural water]. Tyumen, 2012. 168 p. (in Russian).

9. Kastner, M., Hofrichter, M. Biodegradation of humic substances // In: Hofrichter, M., Steinbuchel, A. (eds), Biopolymers Lignin, Humic Substances and Coal. 2001. V. 1. Wiley-VCH. Weinheim. Germany. Pp. 349–378.

10. Hatakka, A. Lignin-modifying enzymes from selected whiterot Fungi — production and role in lignin degradation. FEMS Microbiology Reviews. 1994. V. 13. Pp. 125–135.

11. Hofrichter, M., Ullrich, R. Heme-thiolate haloperoxidases: versatile biocatalysts with biotechnological and environmental significance. Applied Microbiology and Biotechnology. 2006. V. 71. Pp. 276–288.